UPLC-TOF-HRMS refers to Ultra-Performance Liquid Chromatography coupled with Time-of-Flight High-Resolution Mass Spectrometry. It is a powerful analytical technique that combines the high-resolution capabilities of TOF mass spectrometry with the fast separation provided by UPLC. It allows for highly sensitive and accurate analysis of complex samples in various fields, including Lipidomics, metabolomics, proteomics, and environmental analysis.
Lipidomics is a field of research that focuses on studying the complete set of lipids in biological systems. It involves the identification, quantification, and characterization of lipid molecules in cells, tissues, and biofluids. By employing advanced analytical techniques, such as mass spectrometry and nuclear magnetic resonance, lipidomics aims to understand the roles of lipids in various biological processes and diseases. It provides valuable insights into lipid metabolism, biomarker discovery, and potential therapeutic targets, advancing our understanding of lipid-related conditions and their treatments.
Saliva contains small amounts of lipids, including free fatty acids, phospholipids, cholesterol, and triglycerides. These lipids contribute to oral lubrication, taste perception, and the formation of the salivary film, which helps protect against microbial colonization. Their roles in oral health and disease are still being studied.
This application allows comprehensive lipidomic analyses of lipids in saliva.