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Quantitative analysis of alpha-tocopherol in plasma/serum using LC-FLD

AM-327

Tocopherol is a type of vitamin E that is commonly found in various foods, including vegetable oils, nuts, seeds, and leafy green vegetables. It is a potent antioxidant that plays a crucial role in protecting cells from damage caused by free radicals. Tocopherol exists in several different forms, with alpha-tocopherol being the most biologically active and commonly studied form.

In addition to its antioxidant properties, tocopherol is believed to have various health benefits. It may help support cardiovascular health, boost immune function, and have anti-inflammatory effects. Tocopherol is also important for maintaining healthy skin and hair.

Tocopherol, commonly known as vitamin E, can be detected and measured in plasma, which is the liquid component of blood. Tocopherol plays a crucial role as an antioxidant, protecting cells from oxidative damage. Analytical techniques, such as high-performance liquid chromatography (HPLC), coupled with various detection methods like UV-Vis spectrometry or fluorescence detection, can be used to quantify tocopherol levels in plasma samples. This analysis provides valuable information about the concentration and status of tocopherol in the bloodstream, aiding in the assessment of vitamin E status and its potential health effects.

The assay for tocopherol in fish oil utilizes HPLC-FLD (High-Performance Liquid Chromatography with Fluorescence Detection) to accurately quantify the levels of tocopherol present in the sample. This method allows for precise analysis and determination of tocopherol concentration in plasma, serum and blood samples.