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Vitamins

Vitamins are essential micronutrients that humans and animals need in small amounts for various roles throughout the human body. There are thirteen vitamins all together, some water soluble and some lipid soluble. We master them all.

DSC_6735-Vitas-Analytical-Services.jpg – Vitas Analytical Services

Vitamins are divided into two groups: water-soluble (B-vitamins and C vitamin) and fat-soluble vitamins (A, D, E and K). Unlike water-soluble vitamins that need frequent supply, fat-soluble vitamins are stored in the liver and adipose tissues, and are eliminated much more slowly than water-soluble vitamins. In the blood stream, the fat-soluble vitamins are carried on proteins specialized for this task. Retinol (vitamin A) binds to retinol binding protein (RBP), vitamin D and its derivatives bind to vitamin D binding protein (DBP), whereas others like tocopherols (vitamin E) and phylloquinons/menaquinons (vitamin K) are carried by lipoproteins. The water soluble vitamins B and C flows freely dissolved in the water-phase of blood without the need of binding proteins. 

In Vitas we have worked with vitamin analyses for 28 years and our people are experts on the topic. We master a broad range of techniques used for vitamin determination from a variety of sample types, including blood, serum, plasma, tissue, milk, urine, etc.

Vitamins

Our analytical team

Anette Sauve – Vitas Analytical Services

Anette Sauve

Head of Pharma Services

Anne Marte Haug – Vitas Analytical Services

Anne Marte Haug

Senior Analytical Scientist

Thomas Bjellaas – Vitas Analytical Services

Thomas Bjellaas

Senior Research Scientist

Thomas E. Gundersen – Vitas Analytical Services

Thomas E. Gundersen

Chief Executive Officer (CEO)

Erik Zeiner Hansen – Vitas Analytical Services

Erik Zeiner Hansen

Senior Analytical Scientist

Vitamin A

Number Method
AM-551 Quantification of Vitamin K1 and menaquionones (MK4, MK5, MK6, MK7, MK8, MK9) in plasma using LC-MS/MS
AM-519 Quantification of retinol, RBP4 and vitamin D in Mitra type DBS from premature children
AM-514 Quantification of Vitamin D (Vit-D3 and Vit-D2) and Menaquinone-7 (MK-7) in DBS using LC-MSMS
AM-511 Quantification of Vitamin D (Vit-D3 and Vit-D2) and Vitamin A (Retinol) in DBS using LC-MSMS
AM-450 Quantification of carotenoids in yeast cell pellets using LC-UV
AM-434 Quantification of steroids (cortisone, cortisol, testosterone and 25-OH vitamin D3) in dried blood spots and plasma using LC-MS/MS.
AM-430 Quantification of Menaquinone-7 (MK-7) in DBS by LC-MS/MS
AM-421 Quantification of vitamin E in human blood by using HPLC-FLD
AM-416 Quantification of sodium ascorbate in formulation by LC-UV
AM-402 Quantification of 1,25-dihydroxy vitamin D in plasma/serum using IDS EIA kit
AM-400 Quantification of vitamin B1 in human plasma by HPLC-FLD
AM-398 Quantification of 25-OH Vit D, testosteron and retinol in Mitra sticks
AM-397 Determination of 25-OH vit D from Dried Blood Spots by LC-MS/MS
AM-396 Quantification of folate and vitamin B12 in human serum using Monobind ELISA kit
AM-395 Determination of 25-OH vit D from Dried Blood Spots by ELISA kit
AM-376 Quantification of Vitamin C in microalgae using HPLC-UV
AM-339 Simultaneous quantification of carotenoids, vitamin A and vitamin E in dried blood spots (DBS)
AM-338 Simultaneous quantification of carotenoids, vitamin A and vitamin E in plasma
AM-336 Quantification of vitamin C in algae by LC-UV
AM-334 Quantification of vitamin B12 in algae using a microbiological test kit
AM-329 Quantification of carotenoids, chlorophylls, and vitamins A and E in algae
AM-327 Quantitative analysis of alpha-tocopherol in plasma/serum using LC-FLD
AM-326 Quantification of cholecalciferol (Vitamin D3) in algae using LC-MS/MS
AM-312 Quantification of vitamin K2 in food using LLE and LC-FLD
AM-303 Quantification of MK 7 in milk using LC-MS/MS
AM-288 Quantification of vitamin K2 (menaquinone-4/MK-4) in plasma by using LC-FLD
AM-283 Quantitative analysis of tocopherol in concentrated vitamin oil base by HPLC-FLD
AM-277 Quantification of Menaquinone-7 (MK-7) and a prodrug (SYN 4-73) in human serum using LC-MS/MS
AM-275 Quantification of MK-7 in milk using LC-MS/MS
AM-274 Quantification of MK-7 in oil by HPLC-UV
AM-267 Quantification of vitamin B2 in plasma by HPLC-FLD
AM-264 Quantification of bovine vitamin-D binding protein (DBP) in plasma using ELISA
AM-258 Quantification of retinoid metabolites in zebrafish by LC-MS/MS
AM-239 Quantitative analysis of alpha-tocopherol i oil using HPLC-FLD
AM-238 Quantification of vitamin B6 using HPLC-FLD
AM-237 Quantification of MK-7, prodrug 1 and prodrug 2 in rat serum by LC-MS/MS
AM-231 Quantification of 1,25-dihydroxy vitamin D3 with EIA
AM-223 Quantification of vitamin D3 in oil droplets using LC-UV (DAD)
AM-205 Quantification of Vitamin D3 (Vit-25-OH-D3) and Vitamin D2 (Vit-25-OH-D2) and Vitamin D3-epi-25-OH-D3 in plasma using LC-MS/MS
AM-203 Quantification of Vitamin C in plasma by HPLC-UV
AM-201 Quantification of Retinyl esters in human plasma using LC-MS
AM-198 Quantification of alfa-tocopherol i silage by LC-FLD
AM-185 Measurement of vitamin C (ascorbic acid) in muscle tissues by LC with DAD detection
AM-183 Quantification of alfa-tocopherol in muscle by HPLC-fluorescence detection (FLD)
AM-175 Quantification of vitamin B12 in serum/plasma
AM-171 Determination of tocopherol in fish filet by HPLC-fluorescence detection (FLD)
AM-169 Quantification of vitamin K2 - menaquionone 4 (MK4) in human serum by LC-FLD
AM-168 Quantification of Tocopherol (Vitamin E) in fish oil using LC-FLD
AM-166 Quantification of vitamin C in human plasma by LC-UV
AM-165 Quantification of vitamin K1 in capsules by LC-FLD
AM-156 Quantification of vitamin K1 in dried blood spots (DBS) by LC-FLD
AM-155 Quantification of vitamin E in dried blood spots (DBS) by LC-FLD
AM-154 Quantification of Vitamin A (Retinol) in dried blood spots (DBS) using LC-UV
AM-153 Quantification of Vitamin E in plasma using LC-FLD
AM-152 Quantification of Vitamin A (Retinol) in plasma using LC-UV
AM-151 Determination of 22-hydroxy cholesterol in plasma with HPLC-APCI-MS
AM-150 Quantification of Menaquinones (MK4 and MK7) and Phylloquinone (K1) in human/mouse serum and tissue using LC-MS/MS
AM-149 Quantification of all-trans-retinol in plasma using LC-MS/MS
AM-148 Quantification of Vitamin D3 (Vit-25-OH-D3) and Vitamin D2 (Vit-25-OH-D2), Vitamin D3-epi-25-OH-D3 and Vitamin A (retinol) in plasma using LC-MS/MS
AM-143 Quantification of Retinol (Vitamin-A) in oil using LC-UV
AM-135 Quantification of vitamin E in cod liver oil by LC-UV
AM-132 Quantification of 25-OH-D3 and 3-epi-25-OH-D3 in plasma using LC-MS
AM-130 Quantification of vitamin A and vitamin D in cod liver oil by LC-UV
AM-127 Quantification of carotenoids in dried blood spots (DBS) by LC-UV
AM-093 Quantitative determination of vitamin K1 in plasma by LC-FLD
AM-092 Quantitative determination of alfa-tokoferol in plasma by LC-FLD
AM-082 Qantification of Vitamin D3 in capsules using LC-MS/MS
AM-080 Quantification of vitamin C in oil capsules by LC-UV
AM-076 Quantification of D-alpha tocopherol in oil capsules by LC-UV
AM-068 Quantification of vitamin A in dried blood spots (DBS) using LC/MS with APCI detection
AM-066 Quantification of vitamin K2 (MK7) in tablets by LC-UV
AM-055 Quantification of retinoids in tissue by LC-MS
AM-054 Quantification of retinoids in plasma by LC-MS
AM-053 Quantification of menaquinones , vitamin K2 (MK 4-10) in muscle or beef by LC-FLD
AM-052 Quantification of 25-OH-Vitamin D3 and D2 in plasma/serum by HPLC-MS/MS or MS
AM-043 Quantification of vitamin K1 (phylloquinone) in fish oil by HPLC-FLD and electrochemical reduction
AM-037 Quantification of 25-hydroxy vitamin D3 in dried blood spots (DBS) by HPLC-MS/MS
AM-025 Quantification of retinol in milk by HPLC-UV after alkaline hydrolysis of retinyl esters
AM-020 Measurement of vitamin C (Ascorbic acid) in raw milk by LC with DAD detection
AM-019 Quantification of vitamin E in milk by HPLC-FLD
AM-017 Quantification of Menaquinone-7 (MK-7) in human serum using LC-MS/MS
AM-003 Analytical procedure for dissolution of cholecalciferol in tablets by dissolution apparatus with paddle and HPLC
AM-002 Analytical procedures for identity, assay, degradation products in cholecalciferol concentrate (powder form)
AM-001 Analytical procedures for identification, assay, degradation products and UoC of cholecalsiferol in tablets by HPLC

Vitamin B

Vitamin C

Vitamin D

Number Method
AM-519 Quantification of retinol, RBP4 and vitamin D in Mitra type DBS from premature children
AM-514 Quantification of Vitamin D (Vit-D3 and Vit-D2) and Menaquinone-7 (MK-7) in DBS using LC-MSMS
AM-511 Quantification of Vitamin D (Vit-D3 and Vit-D2) and Vitamin A (Retinol) in DBS using LC-MSMS
AM-434 Quantification of steroids (cortisone, cortisol, testosterone and 25-OH vitamin D3) in dried blood spots and plasma using LC-MS/MS.
AM-402 Quantification of 1,25-dihydroxy vitamin D in plasma/serum using IDS EIA kit
AM-398 Quantification of 25-OH Vit D, testosteron and retinol in Mitra sticks
AM-397 Determination of 25-OH vit D from Dried Blood Spots by LC-MS/MS
AM-395 Determination of 25-OH vit D from Dried Blood Spots by ELISA kit
AM-326 Quantification of cholecalciferol (Vitamin D3) in algae using LC-MS/MS
AM-264 Quantification of bovine vitamin-D binding protein (DBP) in plasma using ELISA
AM-231 Quantification of 1,25-dihydroxy vitamin D3 with EIA
AM-223 Quantification of vitamin D3 in oil droplets using LC-UV (DAD)
AM-205 Quantification of Vitamin D3 (Vit-25-OH-D3) and Vitamin D2 (Vit-25-OH-D2) and Vitamin D3-epi-25-OH-D3 in plasma using LC-MS/MS
AM-151 Determination of 22-hydroxy cholesterol in plasma with HPLC-APCI-MS
AM-148 Quantification of Vitamin D3 (Vit-25-OH-D3) and Vitamin D2 (Vit-25-OH-D2), Vitamin D3-epi-25-OH-D3 and Vitamin A (retinol) in plasma using LC-MS/MS
AM-132 Quantification of 25-OH-D3 and 3-epi-25-OH-D3 in plasma using LC-MS
AM-130 Quantification of vitamin A and vitamin D in cod liver oil by LC-UV
AM-082 Qantification of Vitamin D3 in capsules using LC-MS/MS
AM-052 Quantification of 25-OH-Vitamin D3 and D2 in plasma/serum by HPLC-MS/MS or MS
AM-037 Quantification of 25-hydroxy vitamin D3 in dried blood spots (DBS) by HPLC-MS/MS
AM-003 Analytical procedure for dissolution of cholecalciferol in tablets by dissolution apparatus with paddle and HPLC
AM-001 Analytical procedures for identification, assay, degradation products and UoC of cholecalsiferol in tablets by HPLC

Vitamin E

Vitamin K

Number Method
AM-551 Quantification of Vitamin K1 and menaquionones (MK4, MK5, MK6, MK7, MK8, MK9) in plasma using LC-MS/MS
AM-514 Quantification of Vitamin D (Vit-D3 and Vit-D2) and Menaquinone-7 (MK-7) in DBS using LC-MSMS
AM-430 Quantification of Menaquinone-7 (MK-7) in DBS by LC-MS/MS
AM-312 Quantification of vitamin K2 in food using LLE and LC-FLD
AM-303 Quantification of MK 7 in milk using LC-MS/MS
AM-288 Quantification of vitamin K2 (menaquinone-4/MK-4) in plasma by using LC-FLD
AM-277 Quantification of Menaquinone-7 (MK-7) and a prodrug (SYN 4-73) in human serum using LC-MS/MS
AM-275 Quantification of MK-7 in milk using LC-MS/MS
AM-274 Quantification of MK-7 in oil by HPLC-UV
AM-237 Quantification of MK-7, prodrug 1 and prodrug 2 in rat serum by LC-MS/MS
AM-169 Quantification of vitamin K2 - menaquionone 4 (MK4) in human serum by LC-FLD
AM-165 Quantification of vitamin K1 in capsules by LC-FLD
AM-156 Quantification of vitamin K1 in dried blood spots (DBS) by LC-FLD
AM-150 Quantification of Menaquinones (MK4 and MK7) and Phylloquinone (K1) in human/mouse serum and tissue using LC-MS/MS
AM-093 Quantitative determination of vitamin K1 in plasma by LC-FLD
AM-066 Quantification of vitamin K2 (MK7) in tablets by LC-UV
AM-053 Quantification of menaquinones , vitamin K2 (MK 4-10) in muscle or beef by LC-FLD
AM-043 Quantification of vitamin K1 (phylloquinone) in fish oil by HPLC-FLD and electrochemical reduction
AM-017 Quantification of Menaquinone-7 (MK-7) in human serum using LC-MS/MS
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