In biology, lipids are defined as hydrophobic or amphiphilic molecules and constitutes a large diverse group of molecules responsible for numerous biological functions including membrane structures, energy storage, signal transduction and regulation of gene expression. In addition, lipids have also several applications in cosmetic and food industry. 

Vitas can offer a range of analytical services related to lipid analysis. Our services include the whole workflow from the sample preparation, the analytical measurements and the evaluation of the raw data. We can also offer both targeted and untargeted lipidomic approaches using state of the art technology.  Our extensive lipid analytical services include (but not limited) to lipid groups such as;

  • Fatty acids (Free/Total)
  • Glycerolipids (mono-, di-, and triglycerides)
  • Phospholipids (PC, PE, PI, PA, PG, PS and lyso variants)
  • Sphingolipids (Ceramides, Sphingomyelins, S1P etc)
  • Steroids (Sex hormones, fat soluble vitamins, Cholesterol, Cholesterol esters, etc)

Our lipid services can be applied on a range of different sample types. Typical samples analysed are (but not limited to):
  • Blood related samples (Whole blood, plasma, serum, DBS etc)
  • Tissue related samples (Liver, muscle, Cell lines, liposomes, etc)
  • Tear/meibum samples
  • Marine oils (Calanus, Krill, Cod liver, etc)

Contact us to find out more about our lipid analytical services.


Comprehensive lipidomics

The overall aim of comprehensive lipidomic analysis is to gain as much information about the identity and levels of different lipids present in the sample. With our lipidomic platform, the different classes of lipids are well separated and quantified using state-of-the-art technology such as UPLC coupled to high-resolution mass spectrometry. We have experience on applying the comprehensive lipidomic platform on a variety of different sample types. 

Figure: Illustration of the resolution obtained in 2 dimensions. First dimesion: chromatographic separation in time. Second dimenstion: separation as mass-to-charge ratio using high resolution mass spectrometric detection. 

An example of performing comprehensive lipidomics  on blood plasma sample resulting in separation of the different types of lipids is shown in the figure below. 

Comprehensive lipidomics

Figure: A representative chromatogram of the separation of a selection of different lipid classes from blood plasma sample using UPLC coupled to high resolution mass spectrometry.


 Contact us to find out how our technology and experience can be of service