Dried Blood Spots


The collection of whole blood samples on paper, known as dried blood spot (DBS), dates back to the early 1960s in new-born screening for inherited metabolic disorders. DBS offers a number of advantages over conventional blood collection. As a less invasive sampling method, DBS offers simpler sample collection and storage and easier transport, with reduced infection risk of various pathogens, and requires only a few drops of blood.


The DBS blood collection platform is rapidly becoming an important basis for quantitative analysis of biomarkers.

 The QuantifiedSelf movement  is a movement to incorporate technology into data acquisition on aspects of a person's daily life in terms of inputs (e.g. food consumed, quality of surrounding air), states (e.g. mood, arousal, blood oxygen, glucos, lipids and other  biomarkers), and performance (mental and physical).

Such self-monitoring and self-sensing, which combines wearable sensors (EEG, ECG) collection of biosamples in a non or minimal invasive way  and wearable computing, is also known as lifelogging.

As an important provider of the minimally invasive blood collecting plarrform, DBS and analysis of biomarkers from this sample type, Vitas is very well positioned to becom an important and intergral part of this upcomming megatrend.

When DBS is used, a small amount of animal or human blood is collected usually from the fingertip, the heal (new-born) of the ear/tail/hind leg (animals) and spotted onto a collection card (DBS card) that is then air dried, stored, and shipped in a special bag at room temperature to a laboratory for analysis. One or several circles are punched out of the DBS card and analysed using a bio analytical method validated for the biomarker in question.


The benefits of using DBS as a blood collection technique are many:


Possibility of collecting the sample at remote locations like:


•    At home by yourself, family or home healthcare personnel
•    In a classroom situation
•    In the field
•    At the pharmacy
•    At the gym or when training outside
•    During or before and after sport competitions
•    When seeing a nutritionist or a health couch


Additional benefits


•    There is no need to have a doctor present as for venepuncture
•    DBS is a minimally invasive technique with minimal pain
•    Only a few drops of blood is needed
•    Samples can be shipped by regular mail in normal room temperature
•    The long term stability of DBS is usually very good at -20 ⁰C or even at room temperature
 

The DBS collection kit

Vitas offer several types of kits for DBS blood collection. Single kits in a box to be sent home by regular mail to individual persons or sets for larger studies (100-100 000 kits). Please contact us to discuss what would suite your needs best.


Custom assay development

We develop on request assays for most biomarkers. The degree of assay validation is agreed with sponsor.  There are many options for reporting.

Techniques usually applied for DBS biomarker analysis are:

  • HPLC-UV
  • HPLC-MS/MS
  • GC-MS
  • GC-FID
  • ICP-MS
  • ELISA
Typical biomarkers are:

  • Fatty Acids
  • Cholesterol, triglycerides,
  • Vitamins
  • Carotenoids
  • Inflammation markers
  • C-peptide, glucose, CRP, IGF-1, PYY, Insulin, HbA1c, etc
  • Amino acids



Please see examples for allready established DBS assays on the application page (link below) and illustrated DBS collection instructions and video in the right column
 
 
 a3a739780a987b4415bc0baf0353de73.pngEasy to follow instructions with pictures in Norwegian
2Bioindex_DBS_Instructions_DBS_Vitas AS 2014
Easy to follow instructions with pictures in English

Minimaly invasisve blood collection from the fingertip
Easy collection of a few drops of blood on the DBS collection card
Simple air drying and shipping to Vitas by regular mail

Picture1
  Video instructions in English
 
 


Related pages:  Publications  --  Applications