Vitamins

Vitamins are essential micronutrients humans and animals needs in small amounts for various roles throughout the human body. Vitamins are divided into two groups: water-soluble (B-complex vitamins and C vitamins) and fat-soluble vitamins (A, D, E and K). Unlike water-soluble vitamins that need regular replacement in the body, fat-soluble vitamins are stored in the liver and fatty tissues, and are eliminated much more slowly than watersoluble vitamins. In the blood stream the non water soluble vitamins are carried on proteins specilized for this task. Retinol (VitA) on retinol bibinding protein (RBP) , 25-OH-Vitamin D on vitamin binding protein ( DBP) while others like tocopherols (VitE) and phylloquinone ( VitK1) are carried in lipoproteins. The water soluble vitamins B and C flows freely in the blood stream without the need of support.

In Vitas we have worked with vitamin analysis for twenty years and our people are experts on the topic. We master a broad range of techniques used for vitamin determination from a wide range of sample types, including biological samples like blood, serum, plasma, tissue, milk urine etc. Some of these are listed below.

For all our applications please visit the site APPLICATIONS.
 

VITAMIN A
AM-021Quantification of lycopene in tomato based products by HPLC-UV 
AM-023Quantification of carotenoids in plasma by HPLC-UV 
AM-024Quantification of carotenoids in milk by HPLC-UV 
AM-038Carotenoids in dried Blood spots by HPLC-UV 
AM-070Quantification of retinol in mouse liver by normal phase LC-FLD 
AM-073Quantitative determination of Retinol and retinylester in plasma from rats by LC-MS 
AM-074Quantification of carotenoids in oil capsules med LC-UV 
AM-091Quantitative determination of retinol in plasma by LC-UV 
AM-116Quantitative determination of Retinol in dried blood spots (DBS) from humans by LC-MS 
AM-145Quantitative determination of all-trans retinoic acid in plasma by LC-MS/MS 
AM-176Quantification of Tretinoin (all-trans-retinoic acid) in 0.1% Tretinoin cream by LC-UV 
AM-268Quantification of retinol and tocopherol in plasma using LC-UV-FLD 

VITAMIN B
 
 AM-033Measurement of total glutathione (GSH) in plasma by HPLC with fluoremetric detection
 
 
 AM-158Measurement of total glutathione in Red blood cells (RBC) by HPLC with fluoremetric detection
 
 
AM-170Measurement of Homocysteine in plasma or serum by HPLC with fluoremetric detection
 
 
 AM-175Quantification of Vitamin B12 in serum/plasma
 
 
 AM-184Measurement of total glutathione in muscle tissues by HPLC with fluoremetric detection
 
 
 AM-238Quantification of vitamin B6 using HPLC-FLD
 
 

 

VITAMIN C

 
 AM-020 Measurement of vitamin C (Ascorbic acid) in raw milk by LC with DAD detection
 
 
 AM-080Quantification of  Vitamin C in oil capsules by LC-UV
 
 
 AM-166 Quantification of Vitamin C in human plasma by LC-UV
 
 
 AM-185Measurement of vitamin C (Ascorbic acid) in Muscle tissues by LC with DAD detection
 
 

 

VITAMIN D
 
 AM-037 Quantification of 25-OH-Vitamin D3 in Dried Blood Spots by HPLC-MS/MS
 
 
 AM-052 Quantification of 25-OH-Vitamin D3 and D2 in plasma/serum  by HPLC-MS/MS or MS
 
 
 AM-130 Quantification of  Vitamin-A and Vitamin-D in cod liver oil by LC-UV
 
 
 AM-223  Quantification of vitamin D3 in oil droplets using LC-UV (DAD)
 
 
 AM-231 Quantification of 1,25-Dihydroxy Vitamin D3 with EIA 
   
 AM-264 Quantification of Bovine vitamin-D Binding Protein (DBP) in plasma using ELISA
 
 

 

VITAMIN E

 
 AM-019Quantitative determination of Vitamin E in milk by HPLC-FLD
 
 
  AM-135 Quantification of  Vitamin-E in cod liver oil by LC-UV
 
 
 AM-155Quantification of Vitamin E in dried blood spots by LC-FLD
 
 
 AM-283Quantitative analysis of tocopherol in concentrated vitamin oil base by HPLC-FLD
 
 

 

VITAMIN K

 
 AM-043Quantitative determination of  vitamin K1 (phylloquinone) in fish oil by HPLC-FLD and electrochemical reduction
 
 
 AM-053 Quantification of  menaquinones , vitamin K2 (MK 4-10) in muscle or beef by LC-FLD
 
 
 AM-066Quantification of  vitamin K2 (MK7) in tablets by LC-UV
 
 
 AM-093Quantitative determination of vitamin K1 in plasma by LC-FLD
 
 
 AM-165Quantification of Vitamin K1 in dried blood spots by LC-FLD
 
 
 AM-169Quantification of Vitamin K2 - menaquionone4 -MK4 - in human serum by LC-FLD